12/18/2023 0 Comments Types of asepsis![]() Joseph Clarke in 1790, discovered the relationship of puerperal deaths with the poor hygiene of the precincts destined for motherhood, thus ordering the exhaustive cleaning of those enclosures. The pattern of communication between the interior and exterior of the human body began to be felt, with the onset of fevers that normally culminated in the death of the patient. How did the asepsis come about?įrom the seventeenth to the nineteenth century, deaths due to systemic infections after injuries, surgical interventions or in the immediate postpartum period were very numerous. It provides all health care users with all the necessary objects and materials, in optimal conditions for their use. The main objective of asepsis is to reduce the risk of disease transmission in hospital centers, especially in the surgical area. ![]() The term asepsis should not be confused with the term antisepsis, which although they go hand in hand, the latter refers to the disinfection process of an organism previously infected with living pathogenic microorganisms. Therefore, it is the quality of an object to remain free of putrefaction or decomposition. The word asepsis comes from the Greek, where the prefix"a"means"without","sepsis"means"putrefaction"and the suffix"ia"indicates quality. Aseptic refers to the absence of microorganisms or infection. The container is a safe but not completely aseptic model.The principles of asepsis are those procedures or rules applied to the prevention of infection, to ensure that a certain object or space is sterile and free of microorganisms. ![]() Neither the container nor contaminated drainage fluid caused infection in patients. It is questionable that bleach removes contaminating germs. The control culture was negative in all cases.Īll fluids in the containers were contaminated.įorty percent of contaminants were enterobacteriaceae. When samples were taken after 24 hours, recurrence of some germ was seen in 50% of cases. Most common germs included Serratia marcescens, Pseudomonas putida, Streptococcus agalactiae, Enterobacter cloacae, and Staphylococcus epidermidis, all of them identified twice, while all other organisms only occurred once.Įnterobacteriaceae accounted for more than 40% of germs, while the remaining organisms were mainly environmental germs proliferating at room temperature. The container fluid had Gram-negative germs in 5 cases, Gram-positive germs in 3 cases, and both types of germs in one case. Of these, 60% were Gram-negative and 40% Gram-positive organisms. Ten different germs were identified out of the total 15 germs found. The fluid in the container was contaminated by a germ in 5 cases (55.5%), by 2 germs in 2 cases (22.2%), and by more than 2 germs in another 2 cases (22.2%). ![]() Nine cases of patients on APD, whose containers were cleaned daily, were studied. The drainage container was disinfected with diluted bleach. In addition, serial samples were taken from a patient subgroup to see the type of flora and whether this was sensitive to routine disinfection. A manually drained sample was collected in all cases to be used as control. Samples were taken from patients on APD at our unit for Gram staining and microbiological cultures in standard and blood culture media. To ascertain whether the usual method for disinfecting containers is effective. To assess whether this fluid storage method involves an infection risk for patients. To determine the contamination status of the drainage fluid collected in the container. This container is washed daily with diluted sodium hypochlorite (bleach). 1-4 However, drainage fluid is stored in an open container at room temperature. Patients undergoing automated peritoneal dialysis (APD) usually have a lower risk of peritonitis as compared to those subject to CAPD. Asepsis is essential in automated peritoneal dialysis to prevent infectious complications.
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